Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Cryptomeria japonica (Thunb. ex L.f.) D. Don is a commercial tree native to Japan and one of the most important forest species in that country Azores (Portugal). Because quality C. timber, several genetic improvement programs have been performed. Recently, some studies focusing on somatic embryogenesis carried out. However, this species, process uses immature seeds as initial explants, for reason, it not possible achieve maximum gain (100% donor plant). Although made applying organogenesis success adult trees low. For our main goal was optimize micropropagation method by using older than 30 years source plant material. In sense, first experiment, we studied effect different types explants three basal culture media shoot induction; then, two sucrose concentrations light treatments (LEDs versus fluorescent lights) were tested rooting. second effects growth regulators (6-benzylaminopurine, meta-topolin, thidiazuron) induction subsequent phases analyzed. The cultures produced highest number shoots when QL medium (Quoirin Lepoivre, 1977) long (>1.5 cm) used; obtained higher roots they grown under red LED lights. Moreover, root significantly previously induced with meta-topolin.